Molecularly Imprinted Polymers
Molecular imprinting is a process by which selected functional monomers are allowed to self-assemble around a template molecule and subsequently polymerized in the presence of a crosslinker. Once the template molecule is extracted from the resulting polymer, a cavity complementary in shape and functionality is present in the matrix, which will bind molecules identical or closely related to the template.
Molecularly imprinted polymer (MIP) is a polymer with a Â«memoryÂ» of the shape and the functional groups of a template molecule. This material is designed in order to recognize selectively the template molecule used in the imprinting process, even in the presence of compounds with structure and functionality similar to those of the template. Molecularly imprinted polymer then acts essentially as an antibody. High molecular recognition properties can be achieved with these MIPs for a variety of molecules.
MIPs applications: AFFINIMIPÂ® SPE
Molecularly imprinted polymers (MIPs) present an interesting alternative to IAC (immune assay chromatography), and do not suffer from storage limitations and stability problems regarding organic solvents. They are also characterized by a higher capacity than IAC, i.e. a higher amount of a target analyte being retained on MIP when comparing similar amount of sorbent packed in a SPE cartridge.
Thanks to these interesting properties, POLYINTELL has developed AFFINIMIPÂ® SPE, a range of solid phase extraction phases used for sample cleanup applications in food and feed safety analysis, environment analysis, life sciences and pharmaceutical R&D.
For example we have developed sample preparation methods for a broad range of target molecules such as mycotoxins (Patulin, Zearelonone, Ochratoxin A, Fumonisins), drugs (amphetamines), endocrine disrupting compounds on food matrices (cereals, milk, coffee, wine etc...) such as Bisphenol A, Estrogens (Estradiol)...
Publications and literature references :
- Molecularly imprinted polymer solid-phase extraction for detection of zearalenone in cereal sample extracts detection, Analytica Chimica Acta, 672, 15-19, 2010.
- Solid-phase extraction using molecularly imprinted polymers for selective extraction of a mycotoxin in cereals, J. Chrom. A., 1217, 6668-6673, 2010.
- Solid-phase extraction using molecularly imprinted polymer for selective extraction of natural and synthetic estrogens from aqueous samples, Paolo Lucci, Oscar NĂşĂ±ez, M.T. Galceran, Journal of Chromatography A, 2011, Accepted Manuscript
- Interest of molecularly imprinted polymers in the fight against doping. Extraction of tamoxifen and its main metabolite from urine followed by high-performance liquid chromatography with UV detection. J. Chromatography A, 1196-1197, 81-88, 2008.
Patents available upon request: WO07/004197; EP1996633; WO08/132384; WO09/056716; WO10/029504