AFFINIMIP® SPE Zearalenone cartridges

Zearalenone is a mycotoxin produced as a secondary metabolite of various Fusarium fungi. Zearalenone is known to cause estrogenic effects at relatively low levels, including infertility, reduced serum testosterone levels and sperm counts, reduced incidence of pregnancy, and change in progesterone levels. In addition, Zearalenone can delay the breeding process and cost the producer significant economic and physical losses.

In Europe, Regulation (EC) 1126/2007 sets maximum levels for Zearalenone in foodstuffs such as processed cereal-based food and baby foods at 20µg/Kg.

AFFINIMIP® SPE Zearalenone are selective solid phase extraction cartridges that selectively clean and concentrate the toxin prior to analysis by HPLC.

• Ready for use and optimized extraction methodologies adapted to several matrices
• Perfect clean-up system for trace analysis of Zearalenone
• Very simple protocol
• Compliant with European Commission Regulation (EC) 1126/2007

Chromatograms of maize sample spiked with ZON at level of 85 μg Kg-1 before cleanup (blue) and after cleanup with AFFINIMIP® SPE Zearalenone.

• Tested matrices : Extraction methodologies for Zearalenone from Wheat, Maize, cereal-based baby food and rice
• Other matrices : contact us
• Protocol :
  • Loading : Acetonitrile/ Water
  • Washing : Acetonitrile - Water - Acetic Acid
  • Elution : Methanol - Acetic acid
• Detection : HPLC-Fluorescence excitation/emission wavelengths of 275 and 450nm
• Format : 3mL cartridges
• Particle diameter range : 25-80 µm
• Stability : 3 years
• Shipping & Storage : Ambient temperature
• Catalog number : FS100-02 for 25 columns
  FS100-03 for 50 columns

Zearalenone analysis has been carried out on maize (41µg/Kg), rice (41µg/Kg) and cereal-based baby food (10 and 41µg/Kg). Very simple and clean chromatograms were obtained after a clean up with AFFINIMIP® SPE Zearalenone.

Application note AFFINIMIP® SPE Zearalenone Cereal Products and Baby Food

Automated protocol

The combination of AFFINIMIP® SPE Zearalenone cartridges with an automate GILSON GX-271 ASPEC™ shows accurate and reliable analysis with recovery yields higher than 80% on a wheat sample. 

Application note AFFINIMIP® SPE Zearalenone Automated Protocol (GILSON GX271 ASPEC™)

Publication

Molecularly imprinted polymer solid-phase extraction for detection of zearalenone in cereal sample extracts, Analytica Chimica Acta, 672, 15-19, 2010.