AFFINIMIP® SPE Prep GSH Adducts cartridges

Glutathione (GSH) is the major endogenous antioxidant produced by the cells. In ADMET studies, the potential toxicity of a drug candidate can be evaluated by testing the reactive intermediate formation with GSH. A representative reaction involves in vitro incubation of a drug candidate with an excess of GSH in hepatic microsomes or hepatocytes.

For structural analysis such as NMR, the purification and isolation of a sufficient amount of GSH-conjugates can be successfully obtained with AFFINIMIP® SPE Prep GSH Adducts.

Results : Total-ion-current profile obtained after an extraction on AFFINIMIP® SPE Prep GSH Adducts 10g. Purification of an incubation of 10mL containing 5mM of GSH.

GSH Adducts: No GSH and drug candidate observed

• Ready for use and optimized extraction methodologies
• Complete removal of free GSH and tested molecules
• Fast, simple & specific sample preparation method

• Tested matrices : microsomes incubation
• Other matrices : contact us
• Protocol :
  • Loading : Incubation solution /Acetic Acid/Acetonitrile
  • Washing : Acetonitrile/Water/Acetic Acid
  • Elution : Acetonitrile / Water
• Detection : LC-MS Format: cartridges of 10g or 30g
• Particle diameter range : 25-80 µm
• Stability : 1 year
• Shipping & Storage : Ambient temperature
• Catalog number : PH103-01 for 2 columns AFFINIMIP Prep GSH Adducts (10g)
  PH103-02 for 2 columns AFFINIMIP Prep GSH Adducts (30g)