AFFINIMIP® SPE Patulin cartridges

Patulin is a mycotoxin produced by a variety of molds, particularly Aspergillus and Penicillium. It is commonly found in rotting apples, and the amount of patulin in apple products is generally viewed as a measure of the quality of the apples used in production.

Studies have shown that it is genotoxic. Several countries have instituted patulin restrictions in apple products. The World Health Organization, the FDA and the European Union recommend a maximum concentration of 50µg/L in apple juice and 25µk/kg in apple puree. Member countries of the European Union have set maximum allowable levels of patulin at 10μg/kg in apple juice and solid apple products, including apple compote and apple puree, for infants and young children (European Commission Regulation (EC) 1881/2006).

AFFINIMIP® SPE Patulin are selective solid phase extraction cartridges that selectively clean and concentrate the toxin prior to analysis by HPLC.

• Ready for use and optimized extraction protocol
• Perfect clean-up system for trace analysis of Patulin
• Enrichment of the analyte
• Applicable to several apple derived matrices
• Removal of main matrix interferent: 5-Hydroxymethylfurfural (HMF)
• Compliant with European Commission Regulation (EC) 1881/2006

LC-UV Chromatograms obtained after AFFINIMIP®SPE Patulin Clean-up of an apple juice spiked at 10µg/kg with Patulin (Green) or not spiked (Red).

• Tested matrices: Extraction protocols for Patulin from apple juice (clear & cloudy), apple puree, baby food.
• Other matrices: contact us
• Protocol:
  • Loading: Diluted apple juice or apple puree after treatment with pectinase enzyme.
  • Washing: NaHCO3/Water/Diethyl Ether
  • Elution: Ethyl acetate
• Detection: HPLC-UV
• Format: 3mL cartridges
• Particle diameter range : 25-80 µm
• Stability: 3 years
• Shipping & Storage : Ambient temperature
• Catalog number: FS102-02 for 25 cartridges 3mL / FS102-03 for 50 cartridges 3mL
  FS102-02K for 25 cartridges 3mL + 50mL pectinase enzyme solution / FS102-03K for 50 cartridges + 50mL pectinase enzyme solution

Pectinase : enzymatic solution for the clarification of cloudy apple juice or apple puree prior to Patulin analysis extraction.

Apple juice and apple puree spiked with Patulin were tested on a HPLC system after sample cleanup with AFFINIMIP® SPE Patulin. The chromatograms obtained show that the main matrix interferent, 5-Hydroxymethylfurfural (HMF) is removed. Average Recoveries of 90.6% were obtained in apple juice spiked at 40μg/kg and 84% in apple puree spiked at 20μg/kg. Recoveries > 80% were also obtained in baby food at maximum allowable level for infants and young children (10μg/kg).

Learn more : Application note AFFINIMIP® SPE PATULIN Apple Juice and Apple Puree
                     Application note AFFINIMIP® SPE PATULIN Baby Food

Apple-fruit puree

Apple/pear and apple/strawberry purees were tested on a HPLC system after sample cleanup with AFFINIMIP® SPE Patulin. Recoveries above 75% were obtained.

Application note AFFINIMIP® SPE Patulin apple-fruit puree

Cider

Cider spiked with Patulin at 10µg/kg and 40 µg/kg was tested on a HPLC system after sample cleanup with AFFINIMIP® SPE Patulin. Clean chromatograms and recoveries above 80% were obtained.

Application note AFFINIMIP® SPE Patulin in cider

Automated protocol

The combination of AFFINIMIP® SPE Patulin cartridges with an automate GILSON GX-271 ASPEC™ shows accurate and reliable analysis with recovery yields higher than 80% on a apple juice. 

Application note AFFINIMIP® SPE Patulin automated protocol