AFFINIMIP® SPE Ochratoxin A cartridges

Ochratoxin (OTA) is a mycotoxin produced as a secondary metabolite of various Aspergillus and Penicillium fungis. It can be found on several commodities (e.g. cereals and cereal-based products, coffee, beer, grape juice, wine, cacao products).

Ochratoxin A exhibits toxicity in animals and mankind, including nephrotoxic, hepatotoxic, immunotoxic, teratogenic and carcinogenic effects and represents therefore a serious health risk to livestock and population. In order to limit these effects, European Regulation (EC) 1881/2006 sets maximum levels for Ochratoxin A in foodstuffs (e.g. 5ÎĽg/kg in raw cereal grains, 30ÎĽg/kg in spices).

AFFINIMIP® SPE Ochratoxin A are selective solid phase extraction cartridges that selectively clean and concentrate the toxin prior to analysis by HPLC.

• Ready for use and optimized extraction protocols adapted for each tested matrices
• Perfect clean-up system for trace analysis of Ochratoxin A
• Unambiguous and reliable analysis
• Concentration of the analyte
• Compliant with European Commission Regulation (EC) 1881/2006

Fluorescence profile of wheat sample spiked at 2.5 µg/kg before cleanup (red) and after cleanup with AFFINIMIP® SPE Ochratoxin A (blue).

• Tested matrices : Extraction protocols for Ochratoxin A from Wheat, Maize, Pepper, Paprika, red and white Wine.
• Other matrices : contact us
• Protocol :
  • Loading : loading solution
  • Washing : Acetonitrile - HCl solution
  • Elution : Methanol - Acetic acid
• Detection : HPLC-Fluorescence excitation/emission wavelengths of 333 and 460nm
• Format : 3mL cartridges
• Particle diameter range : 25-80 µm
• Stability : 3 years
• Shipping & Storage : Ambient temperature
• Catalog number : FS101-02 for 25 columns
  FS101-03 for 50 columns

Ochratoxin A analysis has been carried out on Wheat and several spices (Paprika, grey Pepper). After cleanup and concentration with AFFINIMIP® SPE Ochratoxin A, recovery yields higher than 90% (concentration: 30µg/Kg) were obtained except for Grey Pepper (73% for a concentration of 5µg/Kg).

Application note AFFINIMIP® SPE Ochratoxin A Cereals and Spices

Red & white wines

Ochratoxin A concentrations in red and white wines were determined after pre-treatment with AFFINIMIP® SPE Ochratoxin A. High recovery yields were obtained via a very simple protocol.

Application note AFFINIMIP® SPE Ochratoxin A Red and White Wines

Automated protocol

The combination of AFFINIMIP® SPE Ochratoxin A SPE cartridges with an automate GILSON GX-271 ASPEC™ shows accurate and reliable analysis (CV 1.7% vs CV 2.5% in manual mode).

Application note AFFINIMIP® SPE Ochratoxin A Automated Protocol (GILSON GX271 ASPEC™)

Publications

Solid-phase extraction using molecularly imprinted polymers for selective extraction of a mycotoxin in cereals, Journal of Chromatography A, 1217, 6668-6673, 2010.

Molecularly imprinted polymer as sorbent in micro-solid phase extraction of ochratoxin A in coffee, grape juice and urine, Tien Ping Lee, Bahruddin Saad, Wejdan Shakir Khayoon, Baharuddin Salleh, Talanta (2010), Accepted manuscript, doi:10.1016/j.talanta.2011.10.021