AFFINIMIP® SPE Metanephrines cartridges

Free Metanephrines (Metanephrine (MN), Normetanephrine (NMN) and 3-Methoxytyramine) are created by the action of Catechol-O-Methyl Transferase (COMT) on Epinephrine, Norepinephrine and Dopamine, respectively. Quantification of plasma free metanephrines is used for the diagnosis of pheochromocytoma and the follow-up of patients. It is analytically challenging for clinical researchers due to its extremely low concentrations in biological matrices.

AFFINIMIP® SPE Metanephrines are selective solid phase extraction cartridges that selectively clean and concentrate the Metanephrines prior to analysis by HPLC.

• Extraction and enrichment of a Free Metanephrines
• Ready for use and optimized extraction protocols adapted to each tested matrix
• Simple and reliable analysis

Mass chromatogram (SIM+) of plasma spiked with 60nM of Metanephrines after clean-up with AFFINIMIP® SPE Metanephrines. MN: Metanephrine (m/z=180); NMN: Normetanephrine (m/z=166); 3MT: 3-Methoxytyramine (m/z=151)

• Tested matrices : Extraction methodologies of Metanephrines for plasma and serum
• Other matrices : contact us
• Protocol :
  • Loading : diluted serum or plasma
  • Washing : Water - Methanol
  • Elution : Methanol - Acetic acid
• Detection : HPLC-UV Format: 3mL cartridges
• Particle diameter range : 25-80 µm
• Stability : 3 years
• Shipping & Storage : Ambient temperature
• Catalog number : DG101-02 for 25 columns
  DG101-03 for 50 columns

Application note

This study describes the solid phase extraction of Metanephrines from plasma sample using a Molecularly Imprinted Polymer (MIP) SPE cartridge developed by POLYINTELL that is specific for Metanephrines (AFFINIMIP® SPE Metanephrines). In addition, a comparison with a Weak Cation Exchange SPE cartridge is shown.

Application note AFFINIMIP® SPE Metanephrines from plasma - Comparison with WCX cartridges