AFFINIMIP® SPE FumoZON cartridges

Zearalenone (ZON) and Fumonisins are mycotoxins produced by Fusarium molds. Zearalenone is known to cause estrogenic effects at relatively low levels, including infertility, reduced serum testosterone levels and sperm counts, reduced incidence of pregnancy, and change in progesterone levels. In addition, Zearalenone can delay the breeding process and cost the producer significant economic and physical losses.

Fumonsins are known to cause animal diseases and is suspected human carcinogens. Fumonisin B1 is the most abundant member of the family of Fumonisins while Fumonisin B2 is more cytotoxic than fumonisin B1.

Several countries have instituted Zearalenone and Fumonisins B1+B2 restrictions in foodstuffs. In Europe, Regulation (EC) 1126/2007 sets maximum levels for Zearalenone at 350µg/kg in unprocessed maize, 100µg/kg in maize for human consumption and 20µg/kg in maize based food for infants and young children. Regarding Fumonisins (B1+B2) maximum allowable levels are 4000µg/kg in unprocessed maize, 1000µg/kg in maize for human consumption, and 200µg/kg in maize based food for infants and young children.

AFFINIMIP® SPE FumoZON are selective solid phase extraction cartridges that clean and concentrate Zearalenone and Fumonisins B1+B2 prior to analysis by HPLC-MS.

• Ready for use and optimized extraction methodology adapted to maize matrices
• Perfect clean-up system for trace analysis of Fumonisins B1+B2 and Zearalenone
• Very simple protocol
• Compliant with European Commission Regulation (EC) 1126/2007

Chromatograms obtained after AFFINIMIP® SPE FumoZON Clean-up of a maize flour spiked at 38µg/kg with Zearalenone, 2408µk/kg with Fumonisin B1 and 630µg/kg with Fumonisin B2.

• Tested matrices : Extraction methodology for maize matrices
• Other matrices : contact us
• Protocol :
  • Loading : Methanol/Acetonitrile/ Water
  • Washing : Acetonitrile - Water
  • Elution : Methanol - Acetic acid
• Detection : HPLC-MS ESI
• Format : 3mL cartridges
• Particle diameter range : 25-80 µm
• Stability : 3 years
• Shipping & Storage : Ambient temperature
• Catalog number : FS109-02 for 25 columns
  FS109-03 for 50 columns

Simultaneous analysis of Zearalenone and Fumonisins has been carried out on maize flour and maize based baby food after cleanup with AFFINIMIP® SPE FumoZON. Strong ion-suppression reduction and recoveries higher than 80% compliant with the performance criteria established by the European Commission Regulation (EC 401/2006) were obtained.

Application note AFFINIMIP® SPE FumoZON in Maize and Maize-based Baby Food