Specific sorbent for the solid phase extraction of Chloramphenicol from products of animal origin and biological matrices
Chloramphenicol: a major concern for human health and a challenge in food safety analysis
Chloramphenicol is a broad-spectrum antibiotic widely used in the world in the past. Several health problems are related to its use. As a consequence, several countries (e.g. U.S.A, E.U, Canada...) have prohibited its use for food-producing animals. However, due to its broad spectrum of activity and its availability, Chloramphenicol is still used in several countries to treat food-producing animals. Therefore, chloramphenicol analysis is still a current affair. As no permitted limit has been established, E.U. has defined a Minimum Required Performance Limit (MRPL) set at 0.3µg/kg for products of animal origin (Commission decision 2003/181/EC).
In addition, food matrices are very complexes and induce ion-suppression phenomena which distort analysis results. For such a low MRPL threshold, a cleanup step is crucial in order to improve the sensitivity, the reliability and the specificity before analysis. It is therefore critical to develop a highly selective and sensitive analytical assay to control and monitor Chloramphenicol residues in difficult matrices such as food stuffs.
How to solve this?
POLYINTELL has developed a new class of intelligent polymers (AFFINIMIP®) based on Molecularly Imprinted Polymers specific to Chloramphenicol used as a powerful technique for sample cleanup and pre-concentration. AFFINIMIP® SPE Chloramphenicol cartridge is a simple, fast, sensitive and selective tool for the extraction of Chloramphenicol from complex matrices.
Benefits
- Clean extracts for Lowered LODs: 0.3ÎĽg/kg Chloramphenicol has been detected in a complex matrix such as honey with a single quadrupole mass spectrometer
- High analyte recoveries >90%
- Good repeatability and reproducibility
- Perfect cleanup: very low background and no interferents at the retention time of Chloramphenicol
- High capacity cartridge: no saturation of the cartridge observed at 2ÎĽg/g
Product Information
- Tested matrices : Extraction protocols for Chloramphenicol from honey.
- Other matrices : contact us:
- Protocol:
- Conditioning: Acetonitrile / Deionized Water
- Loading: Honey dissolved in Water
- Washing 1: Deionized Water/ Acetic Acid / Acetonitrile
- Washing 2: Water/ Ammonia
- Washing 3: DiethylEther
- Elution: Methanol
- Format: 1 mL cartridges / 50mg sorbent
- Particle diameter range : 25-80 µm
- Stability : 3 years
- Shipping & Storage : Ambient temperature
- Catalog number:
- FS110-02A for 25 cartridges 1mL
- FS110-03A for 50 cartridges 1mL
Application notes
Honey matrices
This study describes the solid phase extraction of Chloramphenicol from Honey at Minimum Required Performance Limits (MRPLs) of 0.3µg/kg as defined by European Commission decision 2003/181/EC. We demonstrate in this application note that a reliable quantification of Chloramphenicol at low concentrations using AFFINIMIP® SPE Chloramphenicol and single quadrupole mass spectrometry detection is possible. The results demonstrate the efficiency of the clean-up: high recovery yield (> 90%), a good repeatability and reproducibility were obtained.
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