AFFINIMIP® SPE Catecholamines cartridges

Class-selective extraction of free Catecholamines and its amino metabolites from biological samples: plasma, serum

Catecholamines are naturally secreted molecules in the body derived from the amino acid tyrosine containing catechol and amine groups. Some of them are biogenic amines. The most abundant catecholamines are epinephrine (adrenaline, A), norepinephrine (noradrenaline, NA) and dopamine (DA). They are important markers for the diagnosis and management of tumor diseases of the sympathoadrenal system.

AFFINIMIP® Catecholamines are selective solid phase extraction cartridges that selectively clean and concentrate a broad range of catecholamines prior to analysis by HPLC.

Benefits

• Extraction of a broad range of catecholamines
• Ready for use and optimized extraction protocols adapted to each tested matrix
• Simple and reliable analysis
• Easy sample preparation

UV chromatogram of a serum sample spiked with Catecholamines at 450nM before cleanup (pink) and after cleanup (black) with AFFINIMIP® SPE Catecholamines.
Internal Standard: 4-hydroxy-3-methoxybenzylamine (HMBA) (Courtesy of B. Claude and P. Morin, ICOA, Orléans, France)

Product Information

• Tested matrices : Extraction methodologies of Catecholamines for plasma and serum
• Other matrices : contact us
• Protocol :
  • Loading : diluted serum or plasma
  • Washing : Water - Methanol
  • Elution : Methanol - Acetic acid
• Detection : HPLC-UV Format: 3mL cartridges
• Particle diameter range : 25-80 µm
• Stability : 3 years
• Shipping & Storage : Ambient temperature
• Catalog number : DG100-02 for 25 columns
  DG100-03 for 50 columns