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Selective SPE of Bisphenol A from Liquid Infant Formula at low concentrations using Molecularly...

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Euroresidue VII : May 14th 2012

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AFFINIMIP® SPE Bisphenol A cartridges

Reference : FS106-02
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Specific sorbent for the solid phase extraction of Bisphenol A from a variety of matrices: water, milk / infant formula, canned food...

Bisphenol A (or BPA) is a molecule widely used in industry for the synthesis of polycarbonate plastics and epoxy resins. Polycarbonate plastics are used to make a variety of common products including baby and water bottles. Epoxy resins are used as coatings on the inside of almost all food and beverage cans.

BPA is also an endocrine disruptor, which can mimic the body's own hormones and may lead to negative health effects. The migration of BPA from the packaging to food is the main source of consumers' exposure to BPA. So, the European commission has defined a specific migration limit at a maximum level of 0.6 mg of BPA/kg of food (Directive 2011/8/EU of 28 January 2011). In addition, the directive prohibits the use of BPA to manufacture infant feeding bottles.

AFFINIMIP® SPE Bisphenol A are selective solid phase extraction cartridges that selectively clean and concentrate Bisphenol A prior to analysis by chromatography (LC, GC...).

Benefits

  • Perfect clean-up system suitable for all chromatography techniques: LC, GC, MS...
  • Easy quantification of BPA with fluorescence detection : no derivatization as needed in GC
  • Ready for use and optimized extraction protocols: strong reduction of interferents and matrix effects
  • Enrichment of the analyte
  • 2 grades: polypropylene or glass cartridges

1 bisphenol A

Fluorescence profile of 3mL babymilk (λ exc/em: 230-315nm) spiked at 10 ng/mL with Bisphenol A before cleanup (red) and after cleanup with AFFINIMIP® SPE Bisphenol A (blue), and spiked at 173ng/mL after cleanup (pink).

Product Information

  • Tested matrices : Extraction protocols for Bisphenol A from water, milk (infant formula) or canned food.
  • Other matrices : contact us:
  • Protocol:
    • Loading: matrix
    • Washing: Water/ Acetonitrile
    • Elution: Methanol
  • Format:
    • Polypropylene cartridges 3mL
    • Glass cartridges 3mL with Teflon frits: max. quantity BPA < 5ng/cartridge
  • Particle diameter range : 25-80 µm
  • Stability : 3 years
  • Shipping & Storage : Ambient temperature
  • Catalog number:
    • FS106-02 for 25 polypropylene cartridges 3mL
    • FS106-02G for 25 glass cartridges 3mL
    • FS106-03 for 50 polypropylene cartridges 3mL
    • FS106-03G for 50 glass cartridges 3mL

Application notes

Beer matrices

This study describes the solid phase extraction of Bisphenol A from beer at low concentrations (1 and 2µg/L) using AFFINIMIP® SPE Bisphenol A. HPLC analysis was performed with a flurescence detector. The results demonstrate the efficiency of the clean-up: high recovery yield (> 90%), a good repeatability and reproducibility were obtained.

Application Note AFFINIMIP® SPE for the analysis of Bisphenol A in Beer at low concentrations

Liquid Infant Formula

We demonstrate in this application note that a reliable quantification of Bisphenol A in liquid infant formula using fluorescence detection is possible, even at low concentrations(1 and 2µg/L). The use of AFFINIMIP® SPE Bisphenol A enables to eliminate the tedious derivatization step required by gas chromatography.

Application Note AFFINIMIP® SPE for the analysis of Bisphenol A in Infant Formula at low concentrations

Canned Foods

The tests were performed in canned peas and carrots and canned tuna and salmon. The results demonstrate the efficiency of the clean-up with fluorescence detection: high recovery yield (> 95%), a good repeatability and reproducibility were obtained in all the matrices.This method is also perfectly suitable for clean-up before GC-MS/MS or LC-MS/MS.

Application Note AFFINIMIP® SPE for the analysis of Bisphenol A from Canned Foods at low concentrations


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